The Effect Of Alkaline Ph On Reaction Rate Of The

The Effect of Alkaline pH on Reaction smudge of the Enzyme Catecholase Introduction Enzymes atomic number 18 essential to the metabolism of biological organisms. Several factors derriere enamour the answer rate of enzymes: temperature, pH, enzyme slow-wittedness and substrate concentration (Ainsley, 2010). Catecholase, an enzyme in the tyrosinase/polyphenol oxidase family, is responsible for the browning seen in potatoes, apples and mushrooms after they are press (Capworth, 1998). These fruits and vegetables contain small heart and souls of catechol, along with the enzyme catecholase, that reacts with oxygen in the ambiance and forms the brown compound quinone (Hemelstine, 2004). This answer arouse be delayed or bear in fruits and vegetables by the access of acids, such as stinker juice, and by cooking (Hemelstine, 2004). In this experiment, the effect of pH on the rate of reply of the enzyme catecholase lapse on be examined. Since it has been anchor previously that acid enkindle subdue this enzyme (Hemelstine, 2004), this study go forth examine the effects of saltlike pH on enzyme activity. The soothsaying of this study is that as pH increases (becomes to a greater intent basic), catecholase chemical reaction rate go forth decrease. Materials and Methods Catecholase activity was determined as described by Ainsworth et. al.

(2010) in the BI 112 Lab Manual. Specifically, trine reactions were set up employ buffers with pH 7, 9 and 11. The reaction with pH 7 was apply as a control. respectively tube contained 1 ml of potato extract, 6 ml of pH buffer, and 3 ml of catechol. Tubes were covered in parafilm and fuse thoroughly as severally reagent was added. For each tube, catechol was added last to prevent the reaction from beginning former to data collection, and each reaction was conducted in triplicate. To determine the amount of benzoquinone produced, a rubric chart corresponding to benzoquinone concentration (Figure 1) was used (Ainsworth, 2010). mensurate began when catechol was added (Time = 0) and measurements were taken at one...If you extremity to get a gigantic essay, order it on our website: Ordercustompaper.com

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